9199 vc chir 99021 (R&D Systems)
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95
Structured Review
R&D Systems
9199 vc chir 99021
9199 Vc Chir 99021, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/9199+vc+chir+99021/Recombinant+Human+VEGF-C+Protein/pm32640183-217-300-297
Average 95 stars, based on 128 article reviews
9199 Vc Chir 99021, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/9199+vc+chir+99021/Recombinant+Human+VEGF-C+Protein/pm32640183-217-300-297
Average 95 stars, based on 128 article reviews
9199 vc chir 99021 - by Bioz Stars,
2026-10
95/100 stars
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Recombinant:Article Title: Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays VisuLize Factor VIII Antigen Kit (Lot:AG8-0050) Affinity Biologicals Cat# FVIII-AG Formaldehyde-treated serum albumin, FITC labeled D’Liver Cat# FSA-F Acetylated LDL-AF488 Invitrogen Cat# L23380 E.coli BioParticles-AF594 Invitrogen Cat# E23370 Human CD34 MicroBead Isolation kit Miltenyi Biotec Cat# 130-046-702 Human CD31 MicroBead Isolation kit Miltenyi Biotec Cat# 130-091-935 MS columns Miltenyi Biotec Cat# 130-042-201 LS columns Miltenyi Biotec Cat# 130-042-401 RNAqueous-micro kit with RNase-free DNase treatment Ambion Cat# AM1931 iSCRIPT Bio-Rad Cat# 1798841 QuantiFast SYBR Green PCR mix QIAGEN Cat# 204057 Histological Mouse and Rabbit Detection kit – MACH4 Inter Medico Cat# BC-M4U534L Histological Rabbit Detection kit – IMPRESS-AP Vector Cat# MP5401-15 Histological Developer kit, Warp Red Inter Medico Cat# BC-WR806H Histological Developer kit, DAB DAKO Cat# K3468 10x Genomics Single-Cell 30 V2 Reagents 10x Genomics Single Cell 30 v2 Deposited Data Primary Human Liver scRNaseq MacParland et al., 2018 GEO: GSE115469 hPSC-Venous Angioblast-derived in vivo matured LSECs - scRNaseq This study GEO: GSE131987 Experimental Models: Cell Lines Human ESC: HES2 Reubinoff et al., 2000 RRID: CVCL_D093 Human ESC: HES2:tdRFP Irion et al., 2007 N/A Human ESC: HES2:eGFP Irion et al., 2007 N/A Human ESC: H1-eGFP Davidson et al., 2012 N/A Experimental Models: Organisms/Strains NSG mice: NOD.Cg PrkdcscidIl2rgtm1Wjl/SzJ The Jackson Laboratory; OCI colony Cat# 005557 Oligonucleotides Primers for RT-qPCR This study Table S4 Software and Algorithms FlowJo Tree Star; https://www.flowjo.com Version 10 GraphPad Prism GraphPad Software; https://www. graphpad.com/scientific-software/prism Version 8 ImageJ (FIJI) FIJI (Schindelin et al., 2012); https://imagej. nih.gov/ij/ Version 2.0.0-rc-69/1.52p Aperio Image Scope Leica Biosystems; https://www. leicabiosystems.com/digital-pathology/ manage/aperio-imagescope/ Version 12.4.3 R CRAN https://cran.r-project.org/ Version 3.5 Cell Ranger https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger 10x Genomics, version 2.1.0 Scran https://git.bioconductor.org/ packages/scran Version 1.12.1 (Continued on next page) Cell Stem Cell 27, 1–16.e1–e9, August 6, 2020 e3 Blocking Assay:Article Title: Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical Commercial Assays VisuLize Factor VIII Antigen Kit (Lot:AG8-0050) Affinity Biologicals Cat# FVIII-AG Formaldehyde-treated serum albumin, FITC labeled D’Liver Cat# FSA-F Acetylated LDL-AF488 Invitrogen Cat# L23380 E.coli BioParticles-AF594 Invitrogen Cat# E23370 Human CD34 MicroBead Isolation kit Miltenyi Biotec Cat# 130-046-702 Human CD31 MicroBead Isolation kit Miltenyi Biotec Cat# 130-091-935 MS columns Miltenyi Biotec Cat# 130-042-201 LS columns Miltenyi Biotec Cat# 130-042-401 RNAqueous-micro kit with RNase-free DNase treatment Ambion Cat# AM1931 iSCRIPT Bio-Rad Cat# 1798841 QuantiFast SYBR Green PCR mix QIAGEN Cat# 204057 Histological Mouse and Rabbit Detection kit – MACH4 Inter Medico Cat# BC-M4U534L Histological Rabbit Detection kit – IMPRESS-AP Vector Cat# MP5401-15 Histological Developer kit, Warp Red Inter Medico Cat# BC-WR806H Histological Developer kit, DAB DAKO Cat# K3468 10x Genomics Single-Cell 30 V2 Reagents 10x Genomics Single Cell 30 v2 Deposited Data Primary Human Liver scRNaseq MacParland et al., 2018 GEO: GSE115469 hPSC-Venous Angioblast-derived in vivo matured LSECs - scRNaseq This study GEO: GSE131987 Experimental Models: Cell Lines Human ESC: HES2 Reubinoff et al., 2000 RRID: CVCL_D093 Human ESC: HES2:tdRFP Irion et al., 2007 N/A Human ESC: HES2:eGFP Irion et al., 2007 N/A Human ESC: H1-eGFP Davidson et al., 2012 N/A Experimental Models: Organisms/Strains NSG mice: NOD.Cg PrkdcscidIl2rgtm1Wjl/SzJ The Jackson Laboratory; OCI colony Cat# 005557 Oligonucleotides Primers for RT-qPCR This study Table S4 Software and Algorithms FlowJo Tree Star; https://www.flowjo.com Version 10 GraphPad Prism GraphPad Software; https://www. graphpad.com/scientific-software/prism Version 8 ImageJ (FIJI) FIJI (Schindelin et al., 2012); https://imagej. nih.gov/ij/ Version 2.0.0-rc-69/1.52p Aperio Image Scope Leica Biosystems; https://www. leicabiosystems.com/digital-pathology/ manage/aperio-imagescope/ Version 12.4.3 R CRAN https://cran.r-project.org/ Version 3.5 Cell Ranger https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger 10x Genomics, version 2.1.0 Scran https://git.bioconductor.org/ packages/scran Version 1.12.1 (Continued on next page) Cell Stem Cell 27, 1–16.e1–e9, August 6, 2020 e3 |